Composite

Part:BBa_K4800046:Design

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-08)


pTrc99a-GabT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3850
    Illegal XbaI site found at 3877
    Illegal PstI site found at 3889
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3850
    Illegal PstI site found at 3889
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3850
    Illegal BamHI site found at 3871
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3850
    Illegal XbaI site found at 3877
    Illegal PstI site found at 3889
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3850
    Illegal XbaI site found at 3877
    Illegal PstI site found at 3889
    Illegal AgeI site found at 2897
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4073
    Illegal BsaI site found at 5141
    Illegal SapI.rc site found at 767


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.


Source

None

References